Article in Ciência Rural 25(2) · January with 4 Reads o herpesvírus suíno (PRV, vírus da doença de Aujeszky) têm sido amplamente utilizadas em vários. doença de Aujeszky em sistema de baculovirus. Régia Maria Feltrin IILaboratório de Sanidade, Embrapa Suínos e Aves, Concórdia, SC, Brasil. IIICentro de. CLONING AND EXPRESSION OF AUJESZKY’S DISEASE VIRUS GLYCOPROTEIN E .. vírus da doença de Aujeszky de surtos em suínos no Estado de Santa.

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Thus, the optimal concentration of MgCl2 and primers were 1.

Agarose gel electrophoresis was used to detect PCR products. Each one of the nine tissue field samples from pigs diagnosed as PRV infected based on clinical signs and laboratory methods yielded the corresponding PRV amplified product when analyzed.

PRV specific primers were designed using the Oligo 6. The virus principally affects pigs, which are considered to be the natural host for PRV and the reservoir of the virus in nature, but also infects a broad range of wild and non-porcine mammals with the important exception of higher-order primates 8. Detection of porcine circovirus type 2, porcine parvovirus and porcine pseudorabies virus from pigs with postweaning multisystemic wasting syndrome by multiplex PCR.

The nucleotide sequence amplified in this study corresponds to a bp fragment in the gD gene of the PRV genome Also, the BLAST search against nucleotide databases of different herpesvirus and random nucleotide sequences revealed this region is very specific for PRV genomes.

This was possible due to the high annealing temperature of the primers pair designed and contributes to aujeszkyy reaction efficiency. Open in a separate window. The Sma I restriction endonuclease site was used for additional specificity confirmation of the amplification products.

The analysis directly from clinical samples from naturally infected animals proved the potential usefulness of the method for a rapid disease diagnosis from field cases. Lanes 1 and 3 are amplification products, Lanes 2 and 4 are amplification products after digestion with Sma I. Seven tissue samples from clinically healthy animals were negative for PCR amplification data not shown. In general, PRV infections must be considered in the differential diagnosis of respiratory, reproductive and nervous disorders.

Journal List Braz J Microbiol v. Cell biological and molecular characteristics of pseudorabies virus infections in cell cultures and in pigs with emphasis on the respiratory tract. This assay was based on the amplification of a highly conserved viral gD gene fragment. The polymerase chain reaction PCR is a rapid tool that can be used no only to detect acutely PRV infected pigs suinls it is the recommended test for detect PRV latent infection.


Moreover, the viral genomes of a related herpesvirus and other DNA genome porcine viruses as follow: Oligonucleotide primers and restriction endonuclease selection PRV specific primers were designed using the Oligo 6. Replication in the respiratory tract, central nervous system and reproductive organs is responsible for pathological changes causing different disorders The effect of temperature and oligonucleotide primer length on the specificity and efficiency of amplification by the polymerase chain reaction.

Table 1 Primers designed for the specific amplification of the viral gD glycoprotein gene of the PRV genome. The virus primarily replicates in the respiratory tract, spreads along cranial nerves to the brains and via lymph and blood to internal organs, with the reproductive organs being affected. Primers sequences, voena positions and the size of PCR products suino shown in Table 1. Under typical conditions of intensive swine production, several clinically similar viral diseases can occur which require laboratory soena diagnosis.

Suijos possibility to perform annealing and elongation in one single step of the thermal profile contributed to the specificity and the efficiency of the assay and allowed the use of a very fast PCR program. National Xoena for Biotechnology InformationU. Author information Article notes Copyright and License information Disclaimer. The annealing temperature and number of cycles were determined experimentally. Can J Com Med.

Doença de Aujeszky – Wikipédia, a enciclopédia livre

The polymerase chain df PCR can be used to identify PRV doeena in secretions or organ samples and although some PCR assays for PRV detection with different sensitivities have been dd 37915 there is no standard procedure recommended so far 2.

The analytical sensitivity of the test was consistently observed to be 1. The conventional diagnostic procedure is time-consuming and false-negative results may occur in submissions from latently infected animals. This gene codes for an envelope glycoprotein named gD which plays and important role in binding cellular receptors and is critical for virus replication in different organs Iowa State University Press; The trigeminal ganglion is the most suimos site for virus isolation, although latent virus is usually difficult to culture or even impossible 113 and PCR is the method recommended to detect viral genome present in this site.


A rapid, sensitive and specific PCR-based diagnostic assay to detect pseudorabies virus in clinical samples is provided. Specificity of the PRV amplicons was furthermore confirmed by Sma I restriction endonuclease analysis which generated the two expected fragments of and bp in length Fig.

The isolation and characterization of a strain of infectious bovine rhinotracheitis virus from stillbirth in swine. In addition, aukeszky amplifications were obtained in all the tissue samples, from PRV natural infected pigs, evaluated. Induction and inhibition of apoptosis by pseudorabies virus aujeszy the trigeminal ganglion during acute infection of swine. Since the rapid detection of infected animals would reduce the potential transmission of the viruses to uninfected herds avoiding the spread of the diseases The analysis of tissue homogenate samples from naturally infected animals proved the potential usefulness of the method for a rapid disease diagnosis from field cases.

Cell biological and aumeszky characteristics of pseudorabies virus infections in cell cultures and pigs with emphasis on respiratory tract. Multiplex PCR for the simultaneous detection of pseudorabies virus, porcine cytomegalovirus, and porcine circovirus in pigs.

Doença de Aujeszky

This region was highly conserved suinps all reported genomes as shown by aligning of these sequences. The PCR for PRV genome detection is also an important method in screening pig specimens collected for xenotransplantation to increase the safety of organ transplantation 7 and to detect viral infection in a wide spectrum of species reported to be susceptible to PRV, through either natural or experimental infections 8.

Traditionally, PRV detection is based on direct virus isolation followed by confirmation using immunofluorescence, immunoperoxidase or neutralization tests with specific antiserum 2. Especially HVB1 is an important target for specificity assay because is a related herpesvirus which is known to infect swine BHV-1 4. All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License.

A rapid and accurate diagnosis of PRV infection is important for the initiation of appropriate control strategies.

Published online Sep 1.